Review



strep tactin superflow high capacity resin  (IBA Lifesciences)


Bioz Verified Symbol IBA Lifesciences is a verified supplier
Bioz Manufacturer Symbol IBA Lifesciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    IBA Lifesciences strep tactin superflow high capacity resin
    Strep Tactin Superflow High Capacity Resin, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+Superflow+high+capacity+resin/pm42833606-74-42-46
    Average 95 stars, based on 272 article reviews
    strep tactin superflow high capacity resin - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: An Imidazo[2,1‐b][1,3,4]thiadiazole Derivative Inhibits the Virulence Factor α‐Hemolysin by Blocking the Pullout of Its Stem Domain
    Article Snippet: The protein was expressed in E. coli BL21 (DE3) in ZYM‐5052 auto‐inducing medium [ ] at 20°C for 20–24 h. The cell pellet was resuspended in a buffer containing 20 mM Tris/HCl pH 8.5, 300 mM NaCl, one tablet of complete EDTA‐free protease inhibitor cocktail (Roche), and lysed by sonication. .. The protein was isolated from the supernatant after centrifugation for 1 h at 100,000×g using a self‐packed 10 mL column with Strep‐Tactin Superflow High‐Capacity resin (IBA) and eluted from the column with a single step of 5 mM D‐desthiobiotin. .. Initially, different cell lines, A549 (DSMZ #ACC107), L929 (DSMZ #ACC2), KB3.1 (DSMZ #ACC158), RAW 264.7 (ATCC #TIB‐71), and U937 (DSMZ #ACC5), were tested in the assay for Hla activity, following which U937 cells were selected for further assay optimization.

    Centrifugation:

    Article Title: An Imidazo[2,1‐b][1,3,4]thiadiazole Derivative Inhibits the Virulence Factor α‐Hemolysin by Blocking the Pullout of Its Stem Domain
    Article Snippet: The protein was expressed in E. coli BL21 (DE3) in ZYM‐5052 auto‐inducing medium [ ] at 20°C for 20–24 h. The cell pellet was resuspended in a buffer containing 20 mM Tris/HCl pH 8.5, 300 mM NaCl, one tablet of complete EDTA‐free protease inhibitor cocktail (Roche), and lysed by sonication. .. The protein was isolated from the supernatant after centrifugation for 1 h at 100,000×g using a self‐packed 10 mL column with Strep‐Tactin Superflow High‐Capacity resin (IBA) and eluted from the column with a single step of 5 mM D‐desthiobiotin. .. Initially, different cell lines, A549 (DSMZ #ACC107), L929 (DSMZ #ACC2), KB3.1 (DSMZ #ACC158), RAW 264.7 (ATCC #TIB‐71), and U937 (DSMZ #ACC5), were tested in the assay for Hla activity, following which U937 cells were selected for further assay optimization.

    other:

    Article Title: Noncanonical calcium-independent TRPM4 activation governs intestinal fluid homeostasis
    Article Snippet: GDN (#GDN101 25 GM) was from Anatrace (Maumee, OH, USA).

    Purification:

    Article Title: Modified clostridial neurotoxins as vaccine and conjugate vaccine platforms
    Article Snippet: Cells were broken with a French Press, clarified by centrifugation, and filtered through a 0.45 μm Surfactant-Free Cellulose Acetate membrane (Thermo Fischer). .. Lysates were further purified by tandem gravity-flow chromatography using Ni2+-NTA resin (Qiagen), p-aminobenzamidine-agarose (Sigma), and Strep-tactin Superflow high-capacity resin (IBA-LifeSciences). ..

    Chromatography:

    Article Title: Modified clostridial neurotoxins as vaccine and conjugate vaccine platforms
    Article Snippet: Cells were broken with a French Press, clarified by centrifugation, and filtered through a 0.45 μm Surfactant-Free Cellulose Acetate membrane (Thermo Fischer). .. Lysates were further purified by tandem gravity-flow chromatography using Ni2+-NTA resin (Qiagen), p-aminobenzamidine-agarose (Sigma), and Strep-tactin Superflow high-capacity resin (IBA-LifeSciences). ..

    Affinity Purification:

    Article Title: Electrostatic properties of disordered regions control transcription factor search and pioneer activity
    Article Snippet: .. Twin-Strep affinity purification was performed on ice using 1 mL of Strep-Tactin Superflow high-capacity resin (IBA Lifesciences, 2-1208-010-20ML) according to the standard gravity-flow protocol. ..

    Article Title: Electrostatic properties of disordered regions control transcription factor search and pioneer activity.
    Article Snippet: .. Twin-Strep affinity purification was performed on ice using 1 mL of Strep-Tactin Superflow high capacity resin (IBA Lifesciences, 2-1208-010-20ML) according to the standard gravity-flow protocol. ..

    Protein Enrichment:

    Article Title: Biochemical, structural, and functional characterization of the Nocardia asteroides dihydrofolate reductase: a primary target of anti-nocardiosis treatment.
    Article Snippet: .. The extract was then centrifuged for 10min at 3500 g and the supernatant was spun down for 10min at 15 000 g. For protein enrichment by affinity chromatography, the extracts were then loaded onto a gravity column containing about 500 μL of Strep-Tactin Superflow High-Capacity Resin (IBA Life-sciences) equilibrated with PBS 1X and 10 % glycerol buffer. ..

    Affinity Chromatography:

    Article Title: Biochemical, structural, and functional characterization of the Nocardia asteroides dihydrofolate reductase: a primary target of anti-nocardiosis treatment.
    Article Snippet: .. The extract was then centrifuged for 10min at 3500 g and the supernatant was spun down for 10min at 15 000 g. For protein enrichment by affinity chromatography, the extracts were then loaded onto a gravity column containing about 500 μL of Strep-Tactin Superflow High-Capacity Resin (IBA Life-sciences) equilibrated with PBS 1X and 10 % glycerol buffer. ..



    Similar Products

    95
    IBA Lifesciences strep tactin superflow high capacity resin
    Strep Tactin Superflow High Capacity Resin, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+Superflow+high+capacity+resin/pm42833606-74-42-46
    Average 95 stars, based on 1 article reviews
    strep tactin superflow high capacity resin - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    96
    IBA Lifesciences strep tactin xt superflow high capacity beads
    Strep Tactin Xt Superflow High Capacity Beads, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+XT+4Flow+high+capacity+resin/pm42770701-179-25-30
    Average 96 stars, based on 1 article reviews
    strep tactin xt superflow high capacity beads - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    95
    IBA Lifesciences strep-tactin superflow high capacity resin
    Strep Tactin Superflow High Capacity Resin, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+Superflow+high+capacity+resin/custom%402-1208-002%4042259286
    Average 95 stars, based on 1 article reviews
    strep-tactin superflow high capacity resin - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    95
    IBA Lifesciences tag twinstreptag tst
    Tag Twinstreptag Tst, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+Superflow+high+capacity+resin/us12584916-439-49-57
    Average 95 stars, based on 1 article reviews
    tag twinstreptag tst - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    95
    IBA Lifesciences strep tactin resins
    Strep Tactin Resins, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+Superflow+high+capacity+resin/bio_rxiv__64898__2026__01__26__701829-195-7-9
    Average 95 stars, based on 1 article reviews
    strep tactin resins - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    95
    IBA Lifesciences capturedon strep tactin superflowhigh capacity resin
    Capturedon Strep Tactin Superflowhigh Capacity Resin, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strep+tactin+superflow+high+capacity+resin/Strep-Tactin+Superflow+high+capacity+resin/pm41530121-429-2-6
    Average 95 stars, based on 1 article reviews
    capturedon strep tactin superflowhigh capacity resin - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    Image Search Results